On-Site Training

Customized consultation for Academic, Industry, or Regulatory  Research Units

NeuroRenew, Inc. offers virtual workshops providing training in confocal microscopy and quantitative histology. Recognizing there are circumstances when individualized consultation on research objectives and training provides a better value to research units, NeuroRenew, Inc. also offers the option to arrange customized consultation and training addressing the host institution’s own research resources.  This consultation and training could be virtual, remote access, or on-site (when in-person meetings are possible).

Research unit consultation and training is subject to Dr. Peterson’s availability and can be customized to meet specific needs. Standardized curriculum choices and their content are detailed below. For more information or to request a customized quotation, please email info@neurorenew.com.

Course Descriptions

These descriptions outline general curriculum. It is possible to customize course content to meet your specific research requirements.

Confocal Microscopy- Basic (2 Days)

Day 1 09:00 – 09:55 Lecture 1- Principles of Microscopy
  10:00 – 10:55 Lecture 2- Principles of Fluorescence Detection
  11:00 – 11:55 Lecture 3- Principles of Image Acquisition
  12:00 – 13:30 Lunch Break
  13:30 – 17:00 Directed group tutorial at the confocal microscope
Day 2 09:00 – 17:00 Specific Project Group Tutorials

Confocal Microscopy- Expanded (3 Days)

Day 1 09:00 – 10:00 Overview of Course; Participant Presentations
  10:10 – 11:30 Systematic Sampling in Experimental Design
  11:30 – 13:00 Lunch Break
  13:00 – 14:10 Specimen Preparation and Staining of Tissue for Multiple
Label Detection
  14:20 – 15:35 Optical Design and Microscopic Resolution
  15:45 – 17:00 Principles of Fluorescence Microscopy
Day 2 09:00 – 10:15 Confocal Microscopy and Digital Imaging
  10:30 – 12:00 Design and Operation of the Institution’s Confocal
Microscope
  12:00 – 14:00 Confocal Tutorial- Group A
  15:00 – 17:00 Confocal Tutorial- Group B
Day 3 09:00 – 09:30 Post-Acquisition Processing and Image Analysis
  09:30 – 12:00 Tutorial in Photoshop for Scientific Presentation
  12:00 – 13:00 Lunch Break
  13:00 – 15:00 Confocal Tutorial- Group C
  15:00 – 17:00 Confocal Tutorial- Group D

Stereology Theory (1 Day)

Day 1 09:00 – 10:15 Introduction to Design-Based Stereology
  10:30 – 12:00 Estimation of Cell Number
  12:00 – 13:00 Lunch Break
  13:00 – 14:30 Estimation of Volume and Length
  14:30 – 15:20 Sampling, Efficiency, Variation, and Pitfalls
  15:30 – 17:00 Practicum on the Optical Fractionator

Integration of Confocal Stereology (1 Day)

Day 1 09:00 – 10:15 Designing a Stereological Study
  10:30 – 12:00 Computer-Assisted and Confocal Stereology
  12:00 – 13:00 Lunch Break
  13:00 – 17:00 Practical Issues in Confocal Stereology (modified
to address the Institutional resources)

NeuroRenew, Inc. and Dan Peterson are not affiliated with the short courses offered by Peter Mouton