Confocal Microscopy and Stereology

NeuroRenew, Inc. organizes an annual Practical Workshop in Confocal Microscopy and Stereology. Held in Chicago, this venue provides intensive practical training for up to 30 participants every August. Recognizing there are circumstances when on-site training provides a better value to research units, NeuroRenew, Inc. also offers the option to arrange on-site training and consultation using the host institution’s own research resources.

On-site training is subject to Dr. Peterson’s availability and can be customized to meet specific needs. Standardized curriculum choices and their content and pricing are detailed below. For more information or to request a customized quotation, please email info@neurorenew.com.

Course Descriptions

These descriptions outline general curriculum. It is possible to customize course content to meet your specific research requirements.

Confocal Microscopy- Basic (2 Days)

Day 1	09:00 – 09:55	Lecture 1- Principles of Microscopy
	10:00 – 10:55	Lecture 2- Principles of Fluorescence Detection
	11:00 – 11:55	Lecture 3- Principles of Image Acquisition
	12:00 – 13:30	Lunch Break
	13:30 – 17:00	Directed group tutorial at the confocal microscope
Day 2	09:00 – 17:00	Specific Project Group Tutorials

Confocal Microscopy- Expanded (3 Days)

Day 1	09:00 – 10:00	Overview of Course; Participant Presentations
	10:10 – 11:30	Systematic Sampling in Experimental Design
	11:30 – 13:00	Lunch Break
	13:00 – 14:10	Specimen Preparation and Staining of Tissue for Multiple Label Detection
	14:20 – 15:35	Optical Design and Microscopic Resolution
	15:45 – 17:00	Principles of Fluorescence Microscopy
Day 2	09:00 – 10:15	Confocal Microscopy and Digital Imaging
	10:30 – 12:00	Design and Operation of the Institution’s Confocal Microscope
	12:00 – 14:00	Confocal Tutorial- Group A
	15:00 – 17:00	Confocal Tutorial- Group B
Day 3	09:00 – 09:30	Post-Acquisition Processing and Image Analysis
	09:30 – 12:00	Tutorial in Photoshop for Scientific Presentation
	12:00 – 13:00	Lunch Break
	13:00 – 15:00	Confocal Tutorial- Group C
	15:00 – 17:00	Confocal Tutorial- Group D

Stereology Theory (1 Day)

Day 1	09:00 – 10:15	Introduction to Design-Based Stereology
	10:30 – 12:00	Estimation of Cell Number
	12:00 – 13:00	Lunch Break
	13:00 – 14:30	Estimation of Volume and Length
	14:30 – 15:20	Sampling, Efficiency, Variation, and Pitfalls
	15:30 – 17:00	Practicum on the Optical Fractionator

Integration of Confocal Stereology (1 Day)

Day 1	09:00 – 10:15	Designing a Stereological Study
	10:30 – 12:00	Computer-Assisted and Confocal Stereology
	12:00 – 13:00	Lunch Break
	13:00 – 17:00	Practical Issues in Confocal Stereology (modified to address the Institutional resources)

General Pricing

There is an additional Set-Up Fee ($2,000 within North America; $2,500 outside of North America) that is required for all on-site training. Please contact Dr. Peterson at president@neurorenew.com to discuss your specific needs and to obtain an individualized quotation.

Item	Academic Price	For-Profit Price
Confocal Microscopy- Basic (2 Days)	$3,600	$9,000
Confocal Microscopy- Expanded (3 Days)	$5,400	$13,500
Add Stereology Theory (1 Day)	$1,800	$4,500
Add Integration of Confocal Stereology (1 Day)	$1,800	$4,500
Add Project Consultation (per Day)	$1,800	$4,500

Note: Unlimited enrollment allowed for lecture components; maximum of 20 participants for practical training components

NeuroRenew, Inc. and Dan Peterson are not affiliated with the short courses offered by Peter Mouton